FN Archimer Export Format PT J TI A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) BT AF TARIS, Nicolas BARON, S SHARBEL, Tim SAUVAGE, Christopher BOUDRY, Pierre AS 1:;2:;3:;4:;5:; FF 1:PDG-DOP-DCN-AGSAE-LGP;2:;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP; C1 IFREMER, LGP, F-17390 La Tremblade, France. Lab Analy Genet GENINDEXE, La Rochelle, France. C2 IFREMER, FRANCE LAB ANALY GENET GENINDEXE, FRANCE SI LA TREMBLADE SE PDG-DOP-DCN-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 IF 0.746 TC 27 UR https://archimer.ifremer.fr/doc/2005/publication-1814.pdf LA English DT Article DE ;Oysters;Aquaculture;Parental assignment;High throughput;Larval DNA extraction;Multiplex AB The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or adults of the Pacific oyster, Crassostrea gigas, using a multiplex system of three microsatellite loci. In conjunction with a simple DNA extraction protocol, this method enables high throughput analyses of parentage in C. gigas. Using this method, we successfully determined the parentage of 93% (1224 out of 1318) of the progeny in a factorial cross between 3 females and 10 males. The inability to genotype the remaining 7% was due to DNA degradation of larvae rather than assignment uncertainty. PY 2005 PD MAR SO Aquaculture Research SN 1355-557X PU Blackwell science VL 36 IS 5 UT 000227720800013 BP 516 EP 518 DI 10.1111/j.1365-2109.2004.01208.x ID 1814 ER EF