FN Archimer Export Format
PT J
TI Characterization and variations of organic parameters in teleost fish endolymph during day-night cycle, starvation and stress conditions
BT 
AF GUIBBOLINI, M
   BORELLI, G
   MAYER GOSTAN, N
   PRIOUZEAU, F
   DE PONTUAL, Helene
   ALLEMAND, D
   PAYAN, P
AS 1:1;2:1;3:1;4:1;5:2;6:1,3;7:1;
FF 1:;2:;3:;4:;5:PDG-DOP-DCB-STH-LASAA;6:;7:;
C1 Univ Nice, Fac Sci, INRA, UMR 112,Lab ROSE,UNSA 1112, F-06108 Nice 2, France.
   IFREMER, DRV, RH, Lab Sclerochronol Anim Aquat, F-29280 Plouzane, France.
   Ctr Sci Monaco, MC-98000 Monaco, Monaco.
C2 UNIV NICE, FRANCE
   IFREMER, FRANCE
   CTR SCI MONACO, MONACO
SI BREST
SE PDG-DOP-DCB-STH-LASAA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 1.553
TC 14
UR https://archimer.ifremer.fr/doc/2006/publication-1875.pdf
LA English
DT Article
DE ;Turbot;Trout;Stress;Starvation;Day night cycle;Organic matrix;Otolith calcification;In vitro crystallization;Endolymph
AB The aim of the present work was to examine the modifications of the organic composition of fish endolymph under environmental conditions (day-night cycle, starvation and Cl-2-Stress) known to modify otolith growth. Endolymph electrophoretic patterns were compared. An antibody raised against the trout otolith organic matrix allowed examining the variations of organic matrix precursors in the endolymph under the above conditions. Western blot analysis showed bands around 60-80 kDa. A 50% decrease of immunolabelling was observed during the night whereas increases were seen after starvation (factor 3) or stress (factor 2) suggesting that these variations could be related to the organic matrix deposit. A factor retarding in vitro CaCO3 crystallization (FRC) was shown to co-precipitate with endolymph proteins and its apparent molecular mass (determined by measuring the activity after electro elution of gel electrophoresis) was estimated around 20 kDa. The FRC activity was stable during day-night cycle whereas it decreased by 70% and nearly 100% under starvation and stress respectively. These results suggest that the FRC, although retarding in vitro crystallization, plays a major role in the process of otolith calcification and that the decreases measured after starvation and stress are responsible for the decreases of the otolith growth. The variations of these two parameters (precursors and FRC) could contribute for the changes in the microstructure of the otolith. (c) 2006 Elsevier Inc. All rights reserved.
PY 2006
PD SEP
SO Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
SN 1095-6433
PU Elsevier
VL 145
IS 1
UT 000240421200012
BP 99
EP 107
DI 10.1016/j.cbpa.2006.05.003
ID 1875
ER

EF