FN Archimer Export Format PT J TI Impact of 20 : 4n-6 supplementation on the fatty acid composition and hemocyte parameters of the Pacific oyster Crassostrea gigas BT AF DELAPORTE, Maryse SOUDANT, Philippe MOAL, Jeanne GIUDICELLI, Emilie LAMBERT, Christophe SEGUINEAU, Catherine SAMAIN, Jean-Francois AS 1:1;2:3;3:1;4:1;5:3;6:1,2;7:1; FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:PDG-DOP-DCB-PFOM-PI;4:PDG-DOP-DCB-PFOM-PI;5:;6:;7:PDG-DOP-DCB-PFOM-PI; C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, UMR 6539, F-29280 Plouzane, France. IFREMER, Ctr Brest, UMR Physiol & Ecophysiol Mollusques Marins 100, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE IUEM, FRANCE SI BREST SE PDG-DOP-DCB-PFOM-PI EXTERIEUR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 1.935 TC 51 UR https://archimer.ifremer.fr/doc/2006/publication-1876.pdf LA English DT Article DE ;Hemocyte parameters;Oyster immunity;Arachidonic Acid;Poly unsaturated fatty acids;Nutrition;Bivalve;Crassostrea gigas AB Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 mu g ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by hemocytes from ArA-supplemented oysters. Moreover, the inhibitory effect of Vibrio aestuarianus extracellular products on the adhesive proprieties of hemocytes was lessened in oysters fed ArA-supplemented T-Iso. All changes in oyster hemocyte parameters reported in the present study suggest that ArA and/or eicosanoid metabolites affect oyster hemocyte functions. PY 2006 PD JUL SO Lipids SN 0024-4201 PU The American Oil Chemists' Society VL 41 IS 6 UT 000240086100007 BP 567 EP 576 ID 1876 ER EF