FN Archimer Export Format
PT J
TI Detection of human enteric viruses in shellfish collected in Tunisia
BT 
AF ELAMRI, D
   AOUNI, M
   PARNAUDEAU, Sylvain
   LE GUYADER, Soizick
AS 1:1;2:2;3:3;4:3;
FF 1:;2:;3:PDG-DOP-DCN-EMP-MIC;4:PDG-DOP-DCN-EMP-MIC;
C1 IFREMER, Microbiol Lab, F-44311 Nantes 03, France.
   Inst Natl Sci & Technol Mer, Lab Biodivers & Biotechnol Marine, Tunis, Tunisia.
   Fac Pharm, Lab Malad Transmissibles & Subst Biol Act, Tunis, Tunisia.
C2 IFREMER, FRANCE
   INSTM, TUNISIA
   FAC PHARM, TUNISIA
SI NANTES
SE PDG-DOP-DCN-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 1.593
TC 35
UR https://archimer.ifremer.fr/doc/2006/publication-1881.pdf
LA English
DT Article
DE ;Shellfish;Reverse transcription PCR;Human enteric viruses
AB Aims: The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia.   Methods and Results: Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found positive for enterovirus.   Conclusions: The mean number of positive samples found in this study is in accordance with the data found in the literature, indicating that no real difference exists in this respect among countries studied. A notable exception is HAV, which reflects the epidemiological status of the population.   Significance and Impact of the Study: This study highlights the interest to analyse shellfish samples from different production areas. These data will be helpful to understand virus circulation and to improve shellfish safety. The results, which confirm contamination, necessitate the development of appropriate studies and monitoring in all shellfish-producing countries.
PY 2006
PD OCT
SO Letters in Applied Microbiology
SN 0266-8254
PU Blackwell science
VL 43
IS 4
UT 000240441400008
BP 399
EP 404
DI 10.1111/j.1472-765X.2006.01978.x
ID 1881
ER

EF