FN Archimer Export Format
PT J
TI A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax
BT 
AF BOUTET, Isabelle
   KY, Chin Long
   BONHOMME, Francois
AS 1:1;2:2;3:1;
FF 1:PDG-DOP-DCN-AGSAE-GPIA;2:PDG-DOP-DCM-BOME-LALR;3:;
C1 CNRS, UMR IFREMER 5171 Genome, Stn Mediterraneenne Environm Littoral, F-34200 Sete, France.
   Stn IFREMER Palavas Flots, F-34250 Palavas Les Flots, France.
C2 CNRS, FRANCE
   IFREMER, FRANCE
SI AUTRE
   PALAVAS
   MONTPELLIER
SE PDG-DOP-DCN-AGSAE-GPIA
   PDG-DOP-DCM-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.721
TC 73
UR https://archimer.ifremer.fr/doc/2006/publication-1901.pdf
LA English
DT Article
DE ;Suppression subtractive hybridisation;Carbonic anhydrase;Osmoregulation;MRNA expression;Sea bass
AB Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using suppression subtractive hybridisation, we characterised 586 partial cDNA sequences encoding proteins potentially involved in the metabolism of sea bass acclimated to salt- or freshwater under experimental conditions. Using these results, we first characterised complete genomic sequence of a carbonic anhydrase and then analysed mRNA expression of genes potentially involved in osmoregulation mechanisms (Na+/K+-ATPase, carbonic anhydrase, angiotensin-converting enzyme and claudin-3), cell-cycle regulation (secretagogin) and immune system (nephrosin) in gill and intestine of wild fish from open sea and lagoons. Our analyses indicate a strong tissue- and environmental-dependant expression pattern for all the genes studied. A transcriptomic approach such as described in the present paper provides thus a first description of genes involved in metabolic or structural functions important for coping with environmental salinity variations in a euryhaline fish like the common sea bass D. labrax. It should be supplemented by proteomics to check the direct involvement of the gene products at the protein level, and by polymorphism analyses if one is to understand population or individual fluctuations in acclimation to salinity variation. (c) 2006 Elsevier B.V. All rights reserved.
PY 2006
PD SEP
SO Gene
SN 0378-1119
PU Elsevier
VL 379
IS 0
UT 000240695500005
BP 40
EP 50
DI 10.1016/j.gene.2006.04.011
ID 1901
ER

EF