FN Archimer Export Format
PT J
TI Quantification of Vibrio penaeicida, the etiological agent of Syndrome 93 in New Caledonian shrimp, by real-time PCR using SYBR Green I chemistry
BT 
AF GOARANT, Cyrille
   MERIEN, F
AS 1:2;2:1;
FF 1:PDG-DOP-DCOP-AQNC;2:;
C1 Inst Pasteur Nouvelle Caledonie, Lab Rech Bacteriol, Noumea 98846, New Caledonia.
   IFREMER, Dept Aquaculture Nouvelle Caledonie, Noumea 98846, New Caledonia.
C2 INST PASTEUR, FRANCE
   IFREMER, FRANCE
SI SAINT VINCENT
SE PDG-DOP-DCOP-AQNC
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.442
TC 29
UR https://archimer.ifremer.fr/doc/2006/publication-1903.pdf
LA English
DT Article
DE ;Vibriosis;Vibrio;Real time PCR;Quantification;Mariculture;Extraction techniques
AB Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular typing study, to be the infectious source. This particular epidemiological pattern highlights the major role of the factors that trigger and aggravate the disease in grow-out ponds, where shrimp populations carry the pathogen all year round. In order to gain a better understanding of "Syndrome 93" epidemiology, quantification of V penaeicida both in shrimp and the shrimp farm ecosystem is necessary. This article describes the steps in the successful development of a real-time PCR quantification assay of V penaeicida in shrimp haemolymph, seawater (from ponds or bays) and sediment pore water, including the choice of an accurate extraction technique. The entire detection method; including sample processing, DNA extraction and real-time PCR amplification, can be completed within 4 h. (c) 2006 Elsevier B.V All rights reserved.
PY 2006
PD OCT
SO Journal of Microbiological Methods
SN 0167-7012
PU Elsevier
VL 67
IS 1
UT 000240811700004
BP 27
EP 35
DI 10.1016/j.mimet.2006.02.013
ID 1903
ER

EF