FN Archimer Export Format
PT J
TI Cryopreservation of Crassostrea gigas vesicular cells: Viability and metabolic activity
BT 
AF HANQUET, Anne-Caroline
   KELLNER, K
   HEUDE, C
   NAIMI, Amine
   MATHIEU, M
   PONCET, J.M.
AS 1:1;2:1;3:1;4:1,3;5:1;6:2;
FF 1:;2:;3:;4:PDG-DOP-DCB-PFOM-PI;5:;6:;
C1 Univ Caen, Ifremer Physiol & Ecophysiol Mollus Marins, UMR 100, Lab Biol & Biotechnol Marines,UMR PE2M, F-14032 Caen, France.
   Univ Caen, ISBIO, F-14032 Caen, France.
C2 UNIV CAEN, FRANCE
   UNIV CAEN, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 2.162
TC 15
UR https://archimer.ifremer.fr/doc/2006/publication-1938.pdf
LA English
DT Article
DE ;Glycogen metabolism;Vesicular cells;Cryopreservation;Mollusc;Oyster;Crassostrea gigas
AB Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into liquid nitrogen. In these conditions the percentage of viable cells reached 70% of the control. The glucose metabolism of thawed cells was evaluated using radioactive glucose as a tracer. Immediately after thawing, glucose uptake involving membrane transporters was greatly reduced (24% of control) whereas glucose incorporation into glycogen was less affected (68% of control). (c) 2006 Elsevier Inc. All rights reserved.
PY 2006
PD AUG
SO Cryobiology
SN 0011-2240
PU Elsevier
VL 53
IS 1
UT 000239479200004
BP 28
EP 36
DI 10.1016/j.cryobiol.2006.03.008
ID 1938
ER

EF