Development of a TaqMan PCR assay for the detection of Bonamia species

Type Article
Date 2006-07
Language English
Author(s) Corbeil Serge1, Arzul IsabelleORCID2, Diggles Ben3, Heasman Mike4, Chollet Bruno2, Berthe Franck5, Crane Mark St. J.1
Affiliation(s) 1 : CSIRO, Australian Anim Hlth Lab, Fish Dis Lab, Livestock Ind, Geelong, Vic 3220, Australia.
2 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
3 : DigsFish Serv Pty Ltd, Brisbane, Qld, Australia.
4 : NSW Fisheries, Port Stephens, NSW, Australia.
5 : Univ Prince Edward Isl, Atlantic Vet Coll, Dept Pathol & Microbiol, Charlottetown, PE C1A 4P3, Canada.
Source Diseases of aquatic organisms (0177-5103) (Inter-Research), 2006-07 , Vol. 71 , N. 1 , P. 75-80
WOS© Times Cited 26
Keyword(s) Oyster, Real time TaqMan PCR assay, Bonamia spp.
Abstract The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New Zealand, Europe, Canada, Chile and the USA and therefore demonstrated genus specificity as tested in this study.
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Corbeil Serge, Arzul Isabelle, Diggles Ben, Heasman Mike, Chollet Bruno, Berthe Franck, Crane Mark St. J. (2006). Development of a TaqMan PCR assay for the detection of Bonamia species. Diseases of aquatic organisms, 71(1), 75-80. Open Access version :