FN Archimer Export Format
PT J
TI Development of a TaqMan PCR assay for the detection of Bonamia species
BT 
AF CORBEIL, Serge
   ARZUL, Isabelle
   DIGGLES, Ben
   HEASMAN, Mike
   CHOLLET, Bruno
   BERTHE, Franck
   CRANE, Mark St. J.
AS 1:1;2:2;3:3;4:4;5:2;6:5;7:1;
FF 1:;2:PDG-DOP-DCN-AGSAE-LGP;3:;4:;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-DOP-DCN-AGSAE-LGP;7:;
C1 CSIRO, Australian Anim Hlth Lab, Fish Dis Lab, Livestock Ind, Geelong, Vic 3220, Australia.
   IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   DigsFish Serv Pty Ltd, Brisbane, Qld, Australia.
   NSW Fisheries, Port Stephens, NSW, Australia.
   Univ Prince Edward Isl, Atlantic Vet Coll, Dept Pathol & Microbiol, Charlottetown, PE C1A 4P3, Canada.
C2 CSIRO, AUSTRALIA
   IFREMER, FRANCE
   DIGSFISH SERV PTY LTD, AUSTRALIA
   NSW FISHERIES, AUSTRALIA
   UNIV PRINCE EDWARD ISL, CANADA
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 1.509
TC 26
UR https://archimer.ifremer.fr/doc/2006/publication-1980.pdf
LA English
DT Article
DE ;Oyster;Real time TaqMan PCR assay;Bonamia spp.
AB The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New Zealand, Europe, Canada, Chile and the USA and therefore demonstrated genus specificity as tested in this study.
PY 2006
PD JUN
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 71
IS 1
UT 000240510300008
BP 75
EP 80
ID 1980
ER

EF