In situ QCM DNA-biosensor probe modification

Type Article
Date 2006-12
Language English
Author(s) Lazerges m M1, Perrot H1, Zeghib n N1, Antoine Elisabeth2, Compere ChantalORCID3
Affiliation(s) 1 : Univ Paris 06, CNRS, LISE, F-75252 Paris 05, France.
2 : IFREMER, Ctr Nantes, Nantes 03, France.
3 : IFREMER, Ctr Brest, F-29280 Plouzane, France.
Source Sensors and Actuators B: Chemical (0925-4005) (Elsevier), 2006-12 , Vol. 120 , N. 1 , P. 329-337
DOI 10.1016/j.snb.2006.02.024
WOS© Times Cited 37
Keyword(s) QCM, DNA, Biosensor
Abstract We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected. Kinetics and thermodynamic studies of the DNA-disulfide and modified biosensors indicate that the modified biosensor is as sensitive, selective, fast, renewable and reproducible as the DNA-disulfide biosensor, but with a higher hybridization ratio. This modification method offers wider investigation field and practical and economic advantages to DNA-biosensors based on irreversible immobilization of DNA probes on solid substrate. (c) 2006 Elsevier B.V. All rights reserved.
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