|Author(s)||Lazerges m M1, Perrot H1, Zeghib n N1, Antoine Elisabeth2, Compere Chantal3|
|Affiliation(s)||1 : Univ Paris 06, CNRS, LISE, F-75252 Paris 05, France.
2 : IFREMER, Ctr Nantes, Nantes 03, France.
3 : IFREMER, Ctr Brest, F-29280 Plouzane, France.
|Source||Sensors and Actuators B: Chemical (0925-4005) (Elsevier), 2006-12 , Vol. 120 , N. 1 , P. 329-337|
|WOS© Times Cited||37|
|Keyword(s)||QCM, DNA, Biosensor|
|Abstract||We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected. Kinetics and thermodynamic studies of the DNA-disulfide and modified biosensors indicate that the modified biosensor is as sensitive, selective, fast, renewable and reproducible as the DNA-disulfide biosensor, but with a higher hybridization ratio. This modification method offers wider investigation field and practical and economic advantages to DNA-biosensors based on irreversible immobilization of DNA probes on solid substrate. (c) 2006 Elsevier B.V. All rights reserved.|
Lazerges m M, Perrot H, Zeghib n N, Antoine Elisabeth, Compere Chantal (2006). In situ QCM DNA-biosensor probe modification. Sensors and Actuators B: Chemical, 120(1), 329-337. Publisher's official version : https://doi.org/10.1016/j.snb.2006.02.024 , Open Access version : https://archimer.ifremer.fr/doc/00000/1985/