FN Archimer Export Format
PT J
TI In situ QCM DNA-biosensor probe modification
BT 
AF LAZERGES M, M
   PERROT, H
   ZEGHIB N, N
   ANTOINE, Elisabeth
   COMPERE, Chantal
AS 1:1;2:1;3:1;4:2;5:3;
FF 1:;2:;3:;4:PDG-DOP-DCB-ERT-IC;5:PDG-DOP-DCB-ERT-IC;
C1 Univ Paris 06, CNRS, LISE, F-75252 Paris 05, France.
   IFREMER, Ctr Nantes, Nantes 03, France.
   IFREMER, Ctr Brest, F-29280 Plouzane, France.
C2 UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-ERT-IC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.331
TC 38
UR https://archimer.ifremer.fr/doc/2006/publication-1985.pdf
LA English
DT Article
DE ;QCM;DNA;Biosensor
AB We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected. Kinetics and thermodynamic studies of the DNA-disulfide and modified biosensors indicate that the modified biosensor is as sensitive, selective, fast, renewable and reproducible as the DNA-disulfide biosensor, but with a higher hybridization ratio. This modification method offers wider investigation field and practical and economic advantages to DNA-biosensors based on irreversible immobilization of DNA probes on solid substrate. (c) 2006 Elsevier B.V. All rights reserved.
PY 2006
PD DEC
SO Sensors and Actuators B: Chemical
SN 0925-4005
PU Elsevier
VL 120
IS 1
UT 000241489300048
BP 329
EP 337
DI 10.1016/j.snb.2006.02.024
ID 1985
ER

EF