FN Archimer Export Format PT J TI Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency? BT AF BRIAND, Elisabeth SALMAIN, Michèle HENRY, Jean-Marie PERROT, Hubert COMPERE, Chantal PRADIER, Claire-Marie AS 1:1,6;2:2;3:3;4:4;5:5;6:1; FF 1:;2:;3:;4:;5:PDG-DOP-DCB-ERT-IC;6:; C1 Univ Paris 06, CNRS, UMR 7609, Lab React Surface, F-75252 Paris 05, France. Ecole Natl Super Chim, CNRS, UMR 7576, Lab Chim & Biochim Complexes Mol, F-75231 Paris, France. INRA, Unite Bioadhes & Hyg Mat, F-91300 Massy, France. Lab Interfaces & Syst Electrochim, UPR 15, F-75252 Paris 05, France. IFREMER, Dept Essais & Rech Technol Interface & Capteurs, F-29280 Plouzane, France. C2 UNIV PARIS 06, FRANCE ECOLE NATL SUPER CHIM, FRANCE INRA, FRANCE LAB INTERFACES & SYST ELECTROCHIM, FRANCE IFREMER, FRANCE CNRS, FRANCE SI BREST SE PDG-DOP-DCB-ERT-MS PDG-DOP-DCB-ERT-IC IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 copubli-univ-france IF 4.132 TC 87 UR https://archimer.ifremer.fr/doc/2006/publication-1988.pdf LA English DT Article DE ;QCM;IRRAS;Thiol attachment;Immunosensor;Mixed SAM' s AB Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved. PY 2006 PD SEP SO Biosensors and Bioelectronics SN 0956-5663 PU Elsevier VL 22 IS 3 UT 000241023300016 BP 440 EP 448 DI 10.1016/j.bios.2006.05.018 ID 1988 ER EF