FN Archimer Export Format
PT J
TI Improvement of lipid and phospholipid recoveries from sardine (Sardina pilchardus) viscera using industrial proteases
BT 
AF DUMAY, Justine
   DONNAY-MORENO, Claire
   BARNATHAN, G
   JAOUEN, P
   BERGE, Jean-Pascal
AS 1:1;2:1;3:2;4:3;5:1;
FF 1:PDG-DOP-DCN-STAM;2:PDG-DOP-DCN-STAM;3:;4:;5:PDG-DOP-DCN-STAM;
C1 IFREMER, F-44311 Nantes, France.
   Univ Nantes, Nantes Atlant Univ, SMAB, Fac Pharm, F-44035 Nantes, France.
   CNRS, UMR 6144, GEPEA, Samt Nazaire, France.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   CNRS, FRANCE
SI NANTES
SE PDG-DOP-DCN-STAM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.008
TC 56
UR https://archimer.ifremer.fr/doc/2006/publication-2004.pdf
LA English
DT Article
DE ;Fish protein hydrolysate FPH;Sardine;By product;Lipid;Hydrolysis;Phospholipid
AB Enzymatic hydrolysis of sardine viscera by three broad spectrum proteases was investigated using the pHstat method (24 h, pH 8, 50 degrees C). After hydrolysis, three fractions (sludge, aqueous phase and oily phase) were collected. For each fraction, lipids and phospholipids were quantified and molecular weights of aqueous phase peptides were determined. Under these conditions, the degree of hydrolysis (DH) varied from 1.9% (Flavourzyme), 3.1% (Protamex) and to 3.3% (Alcalase). Dry matter distribution indicated that all hydrolysis performed increased solubilisation of proteins: protein recovery for each hydrolysis ranged from 57.4% to 61.2%. Furthermore, the total lipid content in the liquid parts (oily and aqueous phases) increased (at least 85% of lipids quantified in the raw material are in these phases) which may improve lipid recovery for commercial applications. In addition, these lipids were richer in phospholipids than those extracted by classical chemical extraction, especially after Alcalase hydrolysis. (c) 2006 Elsevier Ltd. All rights reserved.
PY 2006
PD NOV
SO Process Biochemistry
SN 1359-5113
PU Elsevier
VL 41
IS 11
UT 000241474200013
BP 2327
EP 2332
DI 10.1016/j.procbio.2006.04.005
ID 2004
ER

EF