First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway

Type Article
Date 2007
Language English
Author(s) Montagnani Caroline1, Avarre Jean-Christophe2, De Lorgeril Julien2, Quiquand M2, Boulo VivianeORCID2, Escoubas Jean-Michel3
Affiliation(s) 1 : Univ Montpellier 2, INRA, Ecol Microbienne Insectes & Interact Hote Pathoge, UMR 1133, F-34095 Montpellier, France.
2 : IFREMER, Taravao 98719, Tahiti, Fr Polynesia.
3 : Lab Symbioses Trop & Mediterraneennes, UMR113, F-34398 Montpellier, France.
Source Developmental & Comparative Immunology (0145-305X) (Elsevier), 2007 , Vol. 31 , N. 1 , P. 1-11
DOI 10.1016/j.dci.2006.04.002
WOS© Times Cited 32
Keyword(s) Damage associated molecular pattern, Tissue inhibitor of metalloproteinase, Metalloproteinase, Mollusk immunity, Crassostrea gigas, Oyster
Abstract In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and upstream region. A 1-kb genomic DNA fragment flanking the 5'-end of the gene contains several regulatory elements and notably three NF-kappa B binding sites. The potential involvement of these motifs in Cg-timp gene regulation is discussed. (c) 2006 Elsevier Ltd. All rights reserved.
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Montagnani Caroline, Avarre Jean-Christophe, De Lorgeril Julien, Quiquand M, Boulo Viviane, Escoubas Jean-Michel (2007). First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway. Developmental & Comparative Immunology, 31(1), 1-11. Publisher's official version : https://doi.org/10.1016/j.dci.2006.04.002 , Open Access version : https://archimer.ifremer.fr/doc/00000/2094/