First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway
| Type | Article | ||||||||
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| Date | 2007 | ||||||||
| Language | English | ||||||||
| Author(s) | Montagnani Caroline 1, Avarre Jean-Christophe2, de Lorgeril Julien2, Quiquand M2, Boulo Viviane2, Escoubas Jean-Michel3 |
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| Affiliation(s) | 1 : Univ Montpellier 2, INRA, Ecol Microbienne Insectes & Interact Hote Pathoge, UMR 1133, F-34095 Montpellier, France. 2 : IFREMER, Taravao 98719, Tahiti, Fr Polynesia. 3 : Lab Symbioses Trop & Mediterraneennes, UMR113, F-34398 Montpellier, France. |
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| Source | Developmental & Comparative Immunology (0145-305X) (Elsevier), 2007 , Vol. 31 , N. 1 , P. 1-11 | ||||||||
| DOI | 10.1016/j.dci.2006.04.002 | ||||||||
| WOS© Times Cited | 33 | ||||||||
| Keyword(s) | Damage associated molecular pattern, Tissue inhibitor of metalloproteinase, Metalloproteinase, Mollusk immunity, Crassostrea gigas, Oyster | ||||||||
| Abstract | In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and upstream region. A 1-kb genomic DNA fragment flanking the 5'-end of the gene contains several regulatory elements and notably three NF-kappa B binding sites. The potential involvement of these motifs in Cg-timp gene regulation is discussed. (c) 2006 Elsevier Ltd. All rights reserved. | ||||||||
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