Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption

Type Article
Date 2006-11
Language English
Author(s) Le Guyader Soizick1, Bon Fabienne2, Demedici Dario3, Parnaudeau Sylvain1, Bertone Alessandra4, Crudeli Silvia5, Doyle Aoife6, Zidane Mohamed1, Suffredini Elisabetta3, Kohli e Evelyne2, Maddalo Francesco4, Monini Marina5, Gallay Anne6, Pommepuy Monique1, Pothier Pierre2, Ruggeri Franco M.5
Affiliation(s) 1 : IFREMER, Microbiol Lab, F-44311 Nantes 03, France.
2 : Ctr Hosp Univ, Lab Virol & Microbiol Med & Mol, Dijon, France.
3 : Ctr Nazl Qualita Alimenti & Ris Alimentari, Rome, Italy.
4 : Azienda Unita Sanitari Locale, La Spezia, Italy.
5 : Ist Super Sanita, Dipartimento Sanita Alimentare & Anim, Rome, Italy.
6 : Inst Veille Sanitaire, Dept Malad Infect, Unite Infect Enter Alimentaires & Zoonoses, St Maurice, France.
Source Journal of Clinical Microbiology (0095-1137) (American Society for Microbiology.), 2006-11 , Vol. 44 , N. 11 , P. 3878-3882
DOI 10.1128/JCM.01327-06
WOS© Times Cited 186
Keyword(s) Seafood, Contamination, Norovirus, Epidemiology, Virology, Oyster
Abstract An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to rapidly connect the data and to fully interpret the results, since it was not obvious that one food could be the link because of the diversity of the several norovirus strains involved in the different cases. It was also demonstrated that heavy rain was responsible for the accidental contamination of seafood, leading to a concentration of up to hundreds of genomic copies per oyster as detected by real-time RT-PCR.
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Le Guyader Soizick, Bon Fabienne, Demedici Dario, Parnaudeau Sylvain, Bertone Alessandra, Crudeli Silvia, Doyle Aoife, Zidane Mohamed, Suffredini Elisabetta, Kohli e Evelyne, Maddalo Francesco, Monini Marina, Gallay Anne, Pommepuy Monique, Pothier Pierre, Ruggeri Franco M. (2006). Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption. Journal of Clinical Microbiology, 44(11), 3878-3882. Publisher's official version : https://doi.org/10.1128/JCM.01327-06 , Open Access version : https://archimer.ifremer.fr/doc/00000/2096/