TY - JOUR T1 - Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13 degrees N hydrothermal vent field, East Pacific Rise A1 - Moussard,Hélène A1 - Corre,Erwan A1 - Cambon-Bonavita,Marie-Anne A1 - Fouquet,Yves A1 - Jeanthon,Christian AD - CNRS, Biol Stn, UMR 7144, Equipe Phytoplancton Ocean,FR 2424, F-29682 Roscoff, France. AD - Univ Bretagne Occidentale, Univ Europeen Mer, IFREMER, CNRS,Lab Microbiol Environm Extremes, Plouzane, France. AD - IFREMER, Ctr Brest, Dept Geosci Marines, Plouzane, France. UR - https://archimer.ifremer.fr/doc/00000/2110/ DO - 10.1111/j.1574-6941.2006.00192.x KW - FISH KW - Arcobacter KW - Epsilonproteobacteria KW - 16S rRNA gene KW - Biofilm KW - Deep sea hydrothermal vent N2 - Rapid growth of microbial sulphur mats have repeatedly been observed during oceanographic cruises to various deep-sea hydrothermal vent sites. The microorganisms involved in the mat formation have not been phylogenetically characterized, although the production of morphologically similar sulphur filaments by a Arcobacter strain coastal marine has been documented. An in situ collector deployed for 5 days at the 13 degrees N deep-sea hydrothermal vent site on the East Pacific Rise (EPR) was rapidly colonized by a filamentous microbial mat. Microscopic and chemical analyses revealed that the mat consisted of a network of microorganisms embedded in a mucous sulphur-rich matrix. Molecular surveys based on 16S rRNA gene and aclB genes placed all the environmental clone sequences within the Epsilonproteobacteria. Although few 16S rRNA gene sequences were affiliated with that of cultured organisms, the majority was related to uncultured representatives of the Arcobacter group (<= 95% sequence similarity). A probe designed to target all of the identified lineages hybridized with more than 95% of the mat community. Simultaneous hybridizations with the latter probe and a probe specific to Arcobacter spp. confirmed the numerical dominance of Arcobacter-like bacteria. This study provides the first example of the prevalence and ecological significance of free-living Arcobacter at deep-sea hydrothermal vents. Y1 - 2006/12 PB - Blackwell science JF - FEMS Microbiology Ecology SN - 0168-6496 VL - 58 IS - 3 SP - 449 EP - 463 ID - 2110 ER -