FN Archimer Export Format PT J TI Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13 degrees N hydrothermal vent field, East Pacific Rise BT AF MOUSSARD, Hélène CORRE, Erwan CAMBON-BONAVITA, Marie-Anne FOUQUET, Yves JEANTHON, Christian AS 1:2;2:1;3:3;4:3;5:2; FF 1:;2:;3:PDG-DOP-DCB-EEP-LMEE;4:PDG-DOP-DCB-GM-LGM;5:; C1 CNRS, Biol Stn, UMR 7144, Equipe Phytoplancton Ocean,FR 2424, F-29682 Roscoff, France. Univ Bretagne Occidentale, Univ Europeen Mer, IFREMER, CNRS,Lab Microbiol Environm Extremes, Plouzane, France. IFREMER, Ctr Brest, Dept Geosci Marines, Plouzane, France. C2 CNRS, FRANCE UBO, FRANCE IFREMER, FRANCE SI BREST SE PDG-DOP-DCB-EEP-LMEE PDG-DOP-DCB-GM-LGM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 3.157 TC 46 TU Centre national de la recherche scientifique Université Pierre et Marie Curie UR https://archimer.ifremer.fr/doc/2006/publication-2110.pdf LA English DT Article CR PHARE BO L'Atalante DE ;FISH;Arcobacter;Epsilonproteobacteria;16S rRNA gene;Biofilm;Deep sea hydrothermal vent AB Rapid growth of microbial sulphur mats have repeatedly been observed during oceanographic cruises to various deep-sea hydrothermal vent sites. The microorganisms involved in the mat formation have not been phylogenetically characterized, although the production of morphologically similar sulphur filaments by a Arcobacter strain coastal marine has been documented. An in situ collector deployed for 5 days at the 13 degrees N deep-sea hydrothermal vent site on the East Pacific Rise (EPR) was rapidly colonized by a filamentous microbial mat. Microscopic and chemical analyses revealed that the mat consisted of a network of microorganisms embedded in a mucous sulphur-rich matrix. Molecular surveys based on 16S rRNA gene and aclB genes placed all the environmental clone sequences within the Epsilonproteobacteria. Although few 16S rRNA gene sequences were affiliated with that of cultured organisms, the majority was related to uncultured representatives of the Arcobacter group (<= 95% sequence similarity). A probe designed to target all of the identified lineages hybridized with more than 95% of the mat community. Simultaneous hybridizations with the latter probe and a probe specific to Arcobacter spp. confirmed the numerical dominance of Arcobacter-like bacteria. This study provides the first example of the prevalence and ecological significance of free-living Arcobacter at deep-sea hydrothermal vents. PY 2006 PD DEC SO FEMS Microbiology Ecology SN 0168-6496 PU Blackwell science VL 58 IS 3 UT 000242016000014 BP 449 EP 463 DI 10.1111/j.1574-6941.2006.00192.x ID 2110 ER EF