FN Archimer Export Format PT J TI Characterization, chemical modifications and in vitro anticoagulant properties of an exopolysaccharide produced by Alteromonas infernus BT AF COLLIEC-JOUAULT, Sylvia CHEVOLOT, Lionel HELLEY, Dominique RATISKOL, Jacqueline BROS, Andrée SINQUIN, Corinne ROGER, Olivier FISCHER, Anne-Marie AS 1:;2:;3:;4:;5:;6:;7:;8:; FF 1:PDG-DRV-VP-BMM;2:;3:;4:PDG-DRV-VP-BMM;5:;6:PDG-DRV-VP-BMM;7:PDG-DRV-VP-BMM;8:; C1 Univ Paris 13, URM2, IFREMER,CNRS,UMR 7540, Lab Biochim & Mol Marines,Dept Valorisat Produits, F-44311 Nantes 3, France. Univ Paris 05, Hematol Lab, CHU Necker Enfants Malad, INSERM,U428, Paris, France. C2 UNIV PARIS 13, FRANCE UNIV PARIS 05, FRANCE SI NANTES SE PDG-DRV-VP-BMM IN WOS Ifremer jusqu'en 2018 copubli-univ-france IF 2.371 TC 52 UR https://archimer.ifremer.fr/doc/2001/publication-2111.pdf LA English DT Article DE ;Sulfation;Sulfated polysaccharide;Serpin;Heparin like;Depolymerization;Anticoagulant activity;Affinity co electrophoresis AB A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in the 4-O-position and disulfated ones in the 2,3-O-positions. Moreover, the compounds generated by the free radical process were more homogeneous with respect to molecular mass. Also for the first time, the anticoagulant activity of the low-molecular-weight exopolysaccharide fractions is reported. When the fractions obtained after sulfation and depolymerization were compared with heparins, anticoagulant activity was detected in oversulfated fractions, but not in native exopolysaccharide. The free radical depolymerized fraction inhibited thrombin generation in both contact-activated and thromboplastin-activated plasma, showing a prolonged lag phase only in the contact-activated assay. Affinity co-electrophoresis studies suggested that a single population of polysaccharide chains binds to antithrombin and that only a subpopulation strongly interacts with heparin cofactor II. (C) 2001 Elsevier Science B.V. All rights reserved. PY 2001 PD OCT SO Biochimica et Biophysica Acta (BBA) - General Subjects SN 0304-4165 PU Elsevier VL 1528 IS 2-3 UT 000172035200011 BP 141 EP 151 DI 10.1016/S0304-4165(01)00185-4 ID 2111 ER EF