Polysaccharide labelling: impact on structural and biological properties

Type Article
Date 2002-11
Language English
Author(s) Roger Olivier, Colliec-Jouault SylviaORCID, Ratiskol Jacqueline, Sinquin CorinneORCID, Guezennec Jean, Fischer Anne-Marie, Chevolot Lionel
Affiliation(s) IFREMER, Unite Rech Marine 2, Dept Valorisat Prod, Lab Biochim & Mol Marines, F-44311 Nantes 3, France.
Fac Sci Pharmaceut & Biol Paris, INSERM, U428, Lab Hematol Hemostase, F-75270 Paris, France.
Univ Paris 13, Lab Rech Macromol, CNRS, FRE 2314, F-93430 Villetaneuse, France.
Source Carbohydrate Polymers (0144-8617) (Elsevier), 2002-11 , Vol. 50 , N. 3 , P. 273-278
DOI 10.1016/S0144-8617(02)00030-9
WOS© Times Cited 10
Keyword(s) Anticoagulant activity, Reductive amination, Fluorescent tagging, Fucan, GAG, Sulphated polysaccharide
Abstract Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science Ltd. All rights reserved.
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