TY - JOUR T1 - Polysaccharide labelling: impact on structural and biological properties A1 - Roger,Olivier A1 - Colliec-Jouault,Sylvia A1 - Ratiskol,Jacqueline A1 - Sinquin,Corinne A1 - Guezennec,Jean A1 - Fischer,Anne-Marie A1 - Chevolot,Lionel AD - IFREMER, Unite Rech Marine 2, Dept Valorisat Prod, Lab Biochim & Mol Marines, F-44311 Nantes 3, France. AD - Fac Sci Pharmaceut & Biol Paris, INSERM, U428, Lab Hematol Hemostase, F-75270 Paris, France. AD - Univ Paris 13, Lab Rech Macromol, CNRS, FRE 2314, F-93430 Villetaneuse, France. UR - https://archimer.ifremer.fr/doc/00000/2113/ DO - 10.1016/S0144-8617(02)00030-9 KW - Anticoagulant activity KW - Reductive amination KW - Fluorescent tagging KW - Fucan KW - GAG KW - Sulphated polysaccharide N2 - Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science Ltd. All rights reserved. Y1 - 2002/11 PB - Elsevier JF - Carbohydrate Polymers SN - 0144-8617 VL - 50 IS - 3 SP - 273 EP - 278 ID - 2113 ER -