FN Archimer Export Format
PT J
TI Polysaccharide labelling: impact on structural and biological properties
BT 
AF ROGER, Olivier
   COLLIEC-JOUAULT, Sylvia
   RATISKOL, Jacqueline
   SINQUIN, Corinne
   GUEZENNEC, Jean
   FISCHER, Anne-Marie
   CHEVOLOT, Lionel
AS 1:;2:;3:;4:;5:;6:;7:;
FF 1:PDG-DRV-VP-BMM;2:PDG-DRV-VP-BMM;3:PDG-DRV-VP-BMM;4:PDG-DRV-VP-BMM;5:PDG-DRV-VP-BMM;6:;7:;
C1 IFREMER, Unite Rech Marine 2, Dept Valorisat Prod, Lab Biochim & Mol Marines, F-44311 Nantes 3, France.
   Fac Sci Pharmaceut & Biol Paris, INSERM, U428, Lab Hematol Hemostase, F-75270 Paris, France.
   Univ Paris 13, Lab Rech Macromol, CNRS, FRE 2314, F-93430 Villetaneuse, France.
C2 IFREMER, FRANCE
   FAC SCI PHARMACEUT & BIOL PARIS, FRANCE
   UNIV PARIS 13, FRANCE
SI NANTES
   BREST
SE PDG-DRV-VP-BMM
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 1.655
TC 12
UR https://archimer.ifremer.fr/doc/2002/publication-2113.pdf
LA English
DT Article
DE ;Anticoagulant activity;Reductive amination;Fluorescent tagging;Fucan;GAG;Sulphated polysaccharide
AB Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science Ltd. All rights reserved.
PY 2002
PD NOV
SO Carbohydrate Polymers
SN 0144-8617
PU Elsevier
VL 50
IS 3
UT 000177137100007
BP 273
EP 278
DI 10.1016/S0144-8617(02)00030-9
ID 2113
ER

EF