FN Archimer Export Format
PT J
TI Capillary electrophoresis determination of the binding affinity of bioactive sulfated polysaccharides to proteins: study of the binding properties of fucoidan to antithrombin
BT 
AF VARENNE, A
   GAREIL, P
   COLLIEC-JOUAULT, Sylvia
   DANIEL, R
AS 1:;2:;3:;4:;
FF 1:;2:;3:PDG-DRV-VP-BMM;4:;
C1 Ecole Natl Super Chim Paris, CNRS, UMR 7575, Lab Electrochim & Chim Analyt, F-75231 Paris 05, France.
   IFREMER, DRV, VP, BM,Lab Biotechnol & Mol Marines, F-44311 Nantes, France.
   Univ Paris 13, CNRS, UMR 7540, Lab Rech Macromol, F-93430 Villetaneuse, France.
C2 ECOLE NATL SUPER CHIM PARIS, FRANCE
   IFREMER, FRANCE
   UNIV PARIS 13, FRANCE
SI NANTES
SE PDG-DRV-VP-BMM
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 2.174
TC 45
UR https://archimer.ifremer.fr/doc/2003/publication-2114.pdf
LA English
DT Article
DE ;Antithrombin;Fucoidan;Electrophoresis;Protein interaction;Polysaccharides
AB The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to obtain the binding constant for the polysaccharide/antithrombin complex. The results indicate that fucoidan binds to antithrombin in a 1:1 stoichiometry and with an affinity depending on the molecular weight of the polysaccharide. For heparin, the binding constant obtained similarly is in accordance with the literature. This is the first report showing the implementation of a capillary electrophoresis method contributing to the mechanistic understanding of the biological activities of fucoidan and providing evidence for the complex formation between fucoidan and the protein inhibitor of the coagulation antithrombin. (C) 2003 Elsevier Science (USA). All rights reserved.
PY 2003
PD APR
SO Analytical Biochemistry
SN 0003-2697
PU Elsevier
VL 315
IS 2
UT 000182332000002
BP 152
EP 159
DI 10.1016/S0003-2697(02)00687-5
ID 2114
ER

EF