FN Archimer Export Format
PT J
TI Effect of an oversulfated exopolysaccharide on angiogenesis induced by fibroblast growth factor-2 or vascular endothelial growth factor in vitro
BT 
AF MATOU, Sabine
   COLLIEC-JOUAULT, Sylvia
   GALY FAUROUX, Isabelle
   RATISKOL, Jacqueline
   SINQUIN, Corinne
   GUEZENNEC, Jean
   FISCHER, Anne-Marie
   HELLEY, Dominique
AS 1:;2:;3:;4:;5:;6:;7:;8:;
FF 1:PDG-DOP-DCB-BM-BMM;2:PDG-DOP-DCB-BM-BMM;3:;4:PDG-DOP-DCB-BM-BMM;5:PDG-DOP-DCB-BM-BMM;6:PDG-DOP-DCB-BM-BMM;7:;8:;
C1 Univ Paris 05, INSERM, F-75006 Paris, France.
   IFREMER, Nantes, France.
   Hop Europeen Georges Pompidou, Assistance Publ Hop Paris, Paris, France.
C2 UNIV PARIS 05, FRANCE
   IFREMER, FRANCE
   HOP EUROPEEN GEORGES POMPIDOU, FRANCE
SI BREST
   NANTES
SE PDG-DOP-DCB-BM-BMM
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 3.617
TC 37
UR https://archimer.ifremer.fr/doc/2005/publication-2118.pdf
LA English
DT Article
DE ;Matrigel;Collagen gel;Alpha 6 integrin subunit;Angiogenic growth factor;Polysaccharide;Angiogenesis
AB The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6) integrin subunit expression, which was enhanced by 50% in the presence of the growth factors. Indeed, a monoclonal anti-alpha(6) blocking antibody abolished this vascular tube formation. EPS had no effect in any of the experimental conditions, underlying the importance of sulfation in the angiogenic effects of exopolysaccharide. By potentiating the angiogenic activity of FGF-2 and/or VEGF, OS-EPS, which possesses low anticoagulant activity and thus a low hemorrhagic risk, could potentially be used to accelerate vascular wound healing or to promote the growth of collateral blood vessels in ischemic tissues. (C) 2004 Elsevier Inc. All rights reserved.
PY 2005
PD MAR
SO Biochemical Pharmacology
SN 0006-2952
PU Elsevier
VL 69
IS 5
UT 000227371400005
BP 751
EP 759
DI 10.1016/j.bcp.2004.11.021
ID 2118
ER

EF