FN Archimer Export Format
PT J
TI Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes
BT 
AF CHATELIER, Aurelien
   IMBERT, Nathalie
   ZAMBONINO-INFANTE, Jose-Luis
   MCKENZIE, David
   BOIS, P
AS 1:1;2:1;3:2;4:3;5:4;
FF 1:;2:;3:PDG-DOP-DCB-PFOM-ARN;4:;5:;
C1 Univ La Rochelle, Lab Biol & Environm Marin, F-17042 La Rochelle, France.
   IFREMER, INRA, Unite Mixre Nutr Poissons, F-29280 Plouzane, France.
   Danish Inst fisheries Res, N Seas Ctr, Dept Marine Ecol & Aquaculture, DK-9850 Hirtshals, Denmark.
   Univ Poitiers, UMR 6187, CNRS, Inst Physiol & Biol Cellulaire, F-86022 Poitiers, France.
C2 UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
   DIFRES, DENMARK
   UNIV POITIERS, FRANCE
SI LA ROCHELLE
   UNIV-FRANCAISE
   BREST
SE PDG-DOP-DCN-AGSAE-CRELA
   PDG-DOP-DCB-PFOM-ARN
   PDG-DOP-DCN-AGSAE-CREMA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 2.631
TC 7
UR https://archimer.ifremer.fr/doc/2006/publication-2120.pdf
LA English
DT Article
DE ;Fatty acid;Sea bass;Ventricular myocyte;L type calcium channel;Oleic acid
AB The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage dependence of either steady state inactivation or activation curves. Taken together, these results indicate that physiological concentrations of free OA decrease the conductance of the L-type inward current, without altering its properties of selectivity and its voltage dependence. The inhibitory effect of OA upon the L-type calcium channel may translate, in vivo, into a protective effect against arrhythmias induced by Ca2+ overload.
PY 2006
PD OCT
SO Journal of Experimental Biology
SN 0022-0949
PU The Company of Biologists
VL 209
IS 20
UT 000242132100013
BP 4033
EP 4039
DI 10.1242/jeb.02470
ID 2120
ER

EF