Ultrasonic depolymerization of an exopolysaccharide produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid
|Author(s)||Petit Anne-Celine1, 2, Noiret Nicolas1, Guezennec Jean2, Gondrexon N3, Colliec-Jouault Sylvia2|
|Affiliation(s)||1 : Ecole Natl Super Chim Rennes, Inst Chim Rennes, CNRS, UMR 6052, F-35700 Rennes, France.
2 : IFREMER, Lab Biotechnol & Mol Marines, Dept Valorisat Prod, F-44311 Nantes 3, France.
3 : UJF, INPG, Lab Electrochim & Physicochim Mat & Interfaces, Equipe Genie Procedes,CNRS,UMR 5631, F-38402 St Martin Dheres, France.
|Source||Ultrasonics Sonochemistry (1350-4177) (Elsevier), 2007-02 , Vol. 14 , N. 2 , P. 107-112|
|WOS© Times Cited||15|
|Keyword(s)||Exopolysaccharide, Depolymerization, Ultrasound|
|Abstract||Low frequency ultrasound was used to depolymerize a high-molecular-weight exopolysaccharide (EPS) produced by a deep-sea hydrothermal bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. The influence of several parameters was examined including the duration of ultrasonic irradiation, EPS concentration. reaction temperature and volume of the sonicated solution. With the aim of optimizing the depolyrnerization, the native EPS was simultaneously treated with hydrogen peroxide and ultrasound. This study identified the sonication conditions that produce low-molecular-weight derivatives from the native EPS (>10(6) Da) with good reproducibility. (C) 2006 Elsevier B.V. All rights reserved.|