Convenient preparation of picolinyl derivatives from fatty acid esters

Type Article
Date 2006
Language English
Author(s) Dubois Nolwenn1, Barthomeuf Chantal2, Berge Jean-PascalORCID1
Affiliation(s) 1 : IFREMER, Ctr Nantes, Dept Sci & Tech Alimentaires Marines, F-44311 Nantes 03, France.
2 : Univ Auvergne, Fac Pharm, Lab Pharmacognosie & Biotechnol, Clermont Ferrand, France.
Source European Journal of Lipid Science and Technology (1438-7697) (Wiley InterScience), 2006 , Vol. 108 , N. 1 , P. 28-32
DOI 10.1002/ejlt.200501217
WOS© Times Cited 20
Keyword(s) Sensitivity, Sterol esters, Fatty acid methyl esters, Gas chromatography, Mass spectrometry, Picolinyl esters
Abstract It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation. Thus, hexane and water have been added while sodium bicarbonate has been removed in order to lower contamination. Temperature and length of the reaction have then been optimised in order to get 100% derivatisation for different kinds of lipids (45 degrees C and 45 min for FAME). Finally, a comparison of the response factors has confirmed the better sensitivity of the picolinyl derivative against FAME (five times more).
Full Text
File Pages Size Access
publication-2125.pdf 5 102 KB Open access
Top of the page