FN Archimer Export Format PT J TI Convenient preparation of picolinyl derivatives from fatty acid esters BT AF DUBOIS, Nolwenn BARTHOMEUF, Chantal BERGE, Jean-Pascal AS 1:1;2:2;3:1; FF 1:PDG-DOP-DCN-STAM;2:;3:PDG-DOP-DCN-STAM; C1 IFREMER, Ctr Nantes, Dept Sci & Tech Alimentaires Marines, F-44311 Nantes 03, France. Univ Auvergne, Fac Pharm, Lab Pharmacognosie & Biotechnol, Clermont Ferrand, France. C2 IFREMER, FRANCE UNIV AUVERGNE, FRANCE SI NANTES SE PDG-DOP-DCN-STAM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 1.184 TC 20 UR https://archimer.ifremer.fr/doc/2006/publication-2125.pdf LA English DT Article DE ;Sensitivity;Sterol esters;Fatty acid methyl esters;Gas chromatography;Mass spectrometry;Picolinyl esters AB It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation. Thus, hexane and water have been added while sodium bicarbonate has been removed in order to lower contamination. Temperature and length of the reaction have then been optimised in order to get 100% derivatisation for different kinds of lipids (45 degrees C and 45 min for FAME). Finally, a comparison of the response factors has confirmed the better sensitivity of the picolinyl derivative against FAME (five times more). PY 2006 SO European Journal of Lipid Science and Technology SN 1438-7697 PU Wiley InterScience VL 108 IS 1 UT 000235225700005 BP 28 EP 32 DI 10.1002/ejlt.200501217 ID 2125 ER EF