FN Archimer Export Format
PT J
TI Characterization of a defensin from the oyster Crassostrea gigas - Recombinant production, folding, solution structure, antimicrobial activities, and gene expression
BT 
AF GUEGUEN, Yannick
   HERPIN, Amaury
   AUMELAS, André
   GARNIER, Julien
   FIEVET, Julie
   ESCOUBAS, Jean-Michel
   BULET, Philippe
   GONZALEZ, Marcelo
   LELONG, Christophe
   FAVREL, Pascal
   BACHERE, Evelyne
AS 1:6;2:2,5;3:3;4:1;5:6;6:1;7:4;8:6;9:2;10:2;11:6;
FF 1:PDG-DOP-DCN-AGSAE-GPIA;2:;3:;4:;5:PDG-DOP-DCM-PM-APOI;6:;7:;8:;9:;10:;11:PDG-DOP-DCN-AGSAE-GPIA;
C1 Univ Montpellier 2, Infremer, CNRS, UMR 5171, F-34095 Montpellier 5, France.
   Univ Caen, UMR 100 Ifremer, Inst Biol Fondamentale & Appl, Lab Biol & Biotechnol Marines, F-14032 Caen, France.
   Univ Montpellier 1, Ctr Biochim Struct, CNRS, UMR 5048,INSERM U554, F-34090 Montpellier, France.
   Atheris Labs, CH-1233 Geneva, Switzerland.
   SARS Int Care Marine Mol Biol, Ctr High Technol, N-5008 Bergen, Norway.
C2 CNRS, FRANCE
   UNIV CAEN, FRANCE
   UNIV MONTPELLIER, FRANCE
   ATHERIS LABS, SWITZERLAND
   SARS INT CARE MARINE MOL BIOL, NORWAY
   IFREMER, FRANCE
SI MONTPELLIER
   PALAVAS
SE PDG-DOP-DCN-AGSAE-GPIA
   PDG-DOP-DCM-PM-APOI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 5.808
TC 160
UR https://archimer.ifremer.fr/doc/2006/publication-2127.pdf
LA English
DT Article
DE ;Crassostrea gigas;Genomic library;Microbiology;Defensin;Oyster
AB In invertebrates, defensins were found in arthropods and in the mussels. Here, we report for the first time the identification and characterization of a defensin (Cg-Def) from an oyster. Cg-def mRNA was isolated from Crassostrea gigas mantle using an expressed sequence tag approach. To gain insight into potential roles of Cg-Def in oyster immunity, we produced the recombinant peptide in Escherichia coli, characterized its antimicrobial activities, determined its solution structure by NMR spectroscopy, and quantified its gene expression in vivo following bacterial challenge of oysters. Recombinant Cg-Def was active in vitro against Gram-positive bacteria but showed no or limited activities against Gram-negative bacteria and fungi. The activity of Cg-Def was retained in vitro at a salt concentration similar to that of seawater. The Cg-Def structure shares the so-called cystine-stabilized alpha-beta motif (CS-alpha beta) with arthropod defensins but is characterized by the presence of an additional disulfide bond, as previously observed in the mussel defensin (MGD-1). Nevertheless, despite a similar global fold, the Cg-Def and MGD-1 structures mainly differ by the size of their loops and by the presence of two aspartic residues in Cg-Def. Distribution of Cg-def mRNA in various oyster tissues revealed that Cg-def is mainly expressed in mantle edge where it was detected by mass spectrometry analyses. Furthermore, we observed that the Cg-def messenger concentration was unchanged after bacterial challenge. Our results suggest that Cg-def gene is continuously expressed in the mantle and would play a key role in oyster by providing a first line of defense against pathogen colonization.
PY 2006
SO Journal of Biological Inorganic Chemistry
SN 0021-9258
PU American Society for Biochemistry and Molecular Biology
VL 281
IS 1
UT 000234307200040
BP 313
EP 323
DI 10.1074/jbc.M510850200
ID 2127
ER

EF