FN Archimer Export Format PT J TI Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes BT AF DUPERRON, Sebastien FIALA MEDIONI, Aline CAPRAIS, Jean-Claude OLU, Karine SIBUET, Myriam AS 1:1,2,3;2:4;3:2;4:2;5:2; FF 1:PDG-DOP-DCB-EEP-LEP;2:;3:PDG-DOP-DCB-EEP-LEP;4:PDG-DOP-DCB-EEP-LEP;5:PDG-CST; C1 Univ Paris 06, UMR 7138, F-75005 Paris, France. IFREMER, Ctr Brest, Lab Environm Profond, F-29280 Plouzane, France. Max Planck Inst Marine Microbiol, Bremen, Germany. Univ Paris 06, Observ Oceanol, Banyuls sur Mer, France. C2 UNIV PARIS 06, FRANCE IFREMER, FRANCE INST MAX PLANCK (MARINE MICROBIOL), GERMANY UNIV PARIS 06, FRANCE SI BREST BREST,SIEGE SE PDG-DOP-DCB-EEP-LEP PDG-CST IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 3.039 TC 57 UR https://archimer.ifremer.fr/doc/2007/publication-2176.pdf LA English DT Article CR NAUTINIL BO L'Atalante DE ;Eastern Mediterranean;Cold seeps;Lucinoma;Lucinidae;Sulphide oxidizing bacteria;Symbiosis AB Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase alpha subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and delta C-13 values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition. PY 2007 SO FEMS Microbiology Ecology SN 0168-6496 PU Blackwell science VL 59 IS 1 UT 000242784700007 BP 64 EP 70 DI 10.1111/j.1574-6941.2006.00194.x ID 2176 ER EF