Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32

Type Article
Date 2006-10
Language English
Author(s) Labreuche Yannick1, Lambert Christophe2, Soudant Philippe2, Boulo VivianeORCID3, Huvet ArnaudORCID1, Nicolas Jean-Louis1
Affiliation(s) 1 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, F-29280 Plouzane, France.
2 : IFREMER, Ctr Brest, Unite Mixte Rech Physiol & Ecophysiol Mollusques, F-29280 Plouzane, France.
3 : Univ Montpellier 2, GPIA, IFREMER, CNRS,UMII,UMR 5171, F-34095 Montpellier, France.
Source Microbes and Infection (1286-4579) (Elsevier), 2006-10 , Vol. 8 , N. 12-13 , P. 2715-2724
DOI 10.1016/j.micinf.2006.07.020
WOS© Times Cited 132
Keyword(s) Crassostrea gigas, Oyster, Pathogenesis, Vibrio aestuarianus, Bivalve immunity
Abstract The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte phagocytosis and adhesive capabilities were affected during the course of infection. Our results also uncovered a previously-undescribed mechanism used by a Vibrio in the initial stages of host interaction: deregulation of the hemocyte oxidative metabolism by enhancing the production of reactive oxygen species and down-regulating superoxide dismutase (Cg-EcSOD) gene expression. This deregulation may provide an opportunity to the pathogen by impairing hemocyte functions and survival. These findings provide new insights into the cellular and molecular bases of the host-pathogen interactions in C. gigas oyster. (c) 2006 Elsevier Masson SAS. All rights reserved.
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Labreuche Yannick, Lambert Christophe, Soudant Philippe, Boulo Viviane, Huvet Arnaud, Nicolas Jean-Louis (2006). Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32. Microbes and Infection, 8(12-13), 2715-2724. Publisher's official version : https://doi.org/10.1016/j.micinf.2006.07.020 , Open Access version : https://archimer.ifremer.fr/doc/00000/2182/