FN Archimer Export Format PT J TI Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 BT AF LABREUCHE, Yannick LAMBERT, Christophe SOUDANT, Philippe BOULO, Viviane HUVET, Arnaud NICOLAS, Jean-Louis AS 1:1;2:2;3:2;4:3;5:1;6:1; FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:;4:PDG-DOP-DCM-BOME-LALR;5:PDG-DOP-DCB-PFOM-PI;6:PDG-DOP-DCB-PFOM-PI; C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, F-29280 Plouzane, France. IFREMER, Ctr Brest, Unite Mixte Rech Physiol & Ecophysiol Mollusques, F-29280 Plouzane, France. Univ Montpellier 2, GPIA, IFREMER, CNRS,UMII,UMR 5171, F-34095 Montpellier, France. C2 UBO, FRANCE IFREMER, FRANCE UNIV MONTPELLIER, FRANCE SI BREST MONTPELLIER SE PDG-DOP-DCB-PFOM-PI PDG-DOP-DCM-BOME-LALR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 3.127 TC 135 UR https://archimer.ifremer.fr/doc/2006/publication-2182.pdf LA English DT Article DE ;Crassostrea gigas;Oyster;Pathogenesis;Vibrio aestuarianus;Bivalve immunity AB The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte phagocytosis and adhesive capabilities were affected during the course of infection. Our results also uncovered a previously-undescribed mechanism used by a Vibrio in the initial stages of host interaction: deregulation of the hemocyte oxidative metabolism by enhancing the production of reactive oxygen species and down-regulating superoxide dismutase (Cg-EcSOD) gene expression. This deregulation may provide an opportunity to the pathogen by impairing hemocyte functions and survival. These findings provide new insights into the cellular and molecular bases of the host-pathogen interactions in C. gigas oyster. (c) 2006 Elsevier Masson SAS. All rights reserved. PY 2006 PD OCT SO Microbes and Infection SN 1286-4579 PU Elsevier VL 8 IS 12-13 UT 000242671200006 BP 2715 EP 2724 DI 10.1016/j.micinf.2006.07.020 ID 2182 ER EF