FN Archimer Export Format PT J TI Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas BT AF TANGUY, Arnaud BOUTET, Isabelle BOUDRY, Pierre DEGREMONT, Lionel LAROCHE, Jean MORAGA, Dario AS 1:1,2;2:2;3:3;4:3;5:2;6:2; FF 1:;2:PDG-DOP-DCN-AGSAE-GPIA;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:;6:; C1 Equipe Evolut & Genet Populat Marines, UMR 7144, Stn Biol, F-29682 Roscoff, France. Univ Bretagne Occidentale, LEMAR, Lab Sci Environm Marin, Inst Univ European Mer,CNRS,UMR 6539, F-29269 Brest, France. IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. C2 UNIV PARIS 06, FRANCE UBO, FRANCE IFREMER, FRANCE SI AUTRE LA TREMBLADE SE PDG-DOP-DCN-AGSAE-GPIA PDG-DOP-DCN-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.721 TC 11 UR https://archimer.ifremer.fr/doc/2006/publication-2220.pdf LA English DT Article DE ;Gene expression;Xenobiotics;Mollusc;Phosphoglucomutase AB Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA expression in oyster tissues in response to xenobiotics (i.e hydrocarbons and pesticides). The results obtained showed that PGM mRNA expression is mostly up-regulated in the first steps of the response to pollutant exposure and is xenobiotic-dependant. (c) 2006 Elsevier B.V. All rights reserved. PY 2006 PD OCT SO Gene SN 0378-1119 PU Elsevier VL 382 UT 000242050400003 BP 20 EP 27 DI 10.1016/j.gene.2006.06.005 ID 2220 ER EF