FN Archimer Export Format
PT J
TI Immobilization of Protein A on SAMS for the elaboration of immunosensors
BT 
AF BRIAND, Elisabeth
   SALMAIN, Michèle
   COMPERE, Chantal
   PRADIER, Claire-Marie
AS 1:1;2:2;3:3;4:1;
FF 1:;2:;3:PDG-DOP-DCB-ERT-IC;4:;
C1 Univ Paris 06, CNRS, UMR 7609, Lab React Surface, F-75005 Paris, France.
   Ecole Natl Super Chim Paris, CNRS, UMR 7576, Lab Chim & Biochim Complexes Mol, F-75231 Paris 05, France.
   IFREMER, Serv Interfaces & Capteurs, F-29280 Plouzane, France.
C2 UNIV PARIS 06, FRANCE
   ENSCP, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-ERT-MS
   PDG-DOP-DCB-ERT-IC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.789
TC 90
UR https://archimer.ifremer.fr/doc/2006/publication-2222.pdf
LA English
DT Article
DE ;PM IRRAS;Antibody;Protein A;Gold;Self assembled monolayers
AB Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal groups. This criterion appeared decisive for efficient covalent binding of Stapkylococcus aureus Protein A (PrA), a protein that displays high affinity for the constant fragment (Fc) of antibodies of the IgG type from various mammal species. The ability of immobilized Protein A to recognize and bind a model IgG appeared to be optimal for the mixed SAM of MUA and the short-chain, omega-hydroxythiot 6-mercaptohexanol in the proportion 1-3. (c) 2006 Elsevier B.V. All rights reserved.
PY 2006
PD DEC
SO Colloids and Surfaces B: Biointerfaces
SN 0927-7765
PU Elsevier
VL 53
IS 2
UT 000243231300013
BP 215
EP 224
DI 10.1016/j.colsurfb.2006.09.010
ID 2222
ER

EF