|Author(s)||Batista Frederico2, 3, Arzul Isabelle1, Pepin Jean-Francois1, Ruano Francisco4, Friedman Carolyn S.5, Boudry Pierre1, Renault Tristan1|
|Affiliation(s)||1 : IFREMER, LGP, F-17390 La Tremblade, France.
2 : INIAP, IPIMAR, CRIPSul, P-8700305 Olhao, Portugal.
3 : Univ Porto, ICBAS, P-4099003 Oporto, Portugal.
4 : INIAP, IPIMAR, Dept Aqu, P-1449006 Lisbon, Portugal.
5 : Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA.
|Source||Journal of Virological Methods (0166-0934) (Elsevier), 2007 , Vol. 139 , N. 1 , P. 1-11|
|WOS© Times Cited||43|
|Keyword(s)||Oyster, Bivalve molluscs, PCR, Detection, OsHV 1, Herpesvirus|
|Abstract||Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1 variant. The literature on DNA extraction methods, primers, PCR strategies, and confirmatory procedures used for the detection and identification of herpesviruses that infect bivalve molluscs are reviewed. (c) 2006 Elsevier B.V. All rights reserved.|
Batista Frederico, Arzul Isabelle, Pepin Jean-Francois, Ruano Francisco, Friedman Carolyn S., Boudry Pierre, Renault Tristan (2007). Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review. Journal of Virological Methods, 139(1), 1-11. Publisher's official version : https://doi.org/10.1016/j.jviromet.2006.09.005 , Open Access version : https://archimer.ifremer.fr/doc/00000/2322/