Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review

Type Article
Date 2007
Language English
Author(s) Batista Frederico2, 3, Arzul IsabelleORCID1, Pepin Jean-Francois1, Ruano Francisco4, Friedman Carolyn S.5, Boudry PierreORCID1, Renault Tristan1
Affiliation(s) 1 : IFREMER, LGP, F-17390 La Tremblade, France.
2 : INIAP, IPIMAR, CRIPSul, P-8700305 Olhao, Portugal.
3 : Univ Porto, ICBAS, P-4099003 Oporto, Portugal.
4 : INIAP, IPIMAR, Dept Aqu, P-1449006 Lisbon, Portugal.
5 : Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA.
Source Journal of Virological Methods (0166-0934) (Elsevier), 2007 , Vol. 139 , N. 1 , P. 1-11
DOI 10.1016/j.jviromet.2006.09.005
WOS© Times Cited 42
Keyword(s) Oyster, Bivalve molluscs, PCR, Detection, OsHV 1, Herpesvirus
Abstract Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1 variant. The literature on DNA extraction methods, primers, PCR strategies, and confirmatory procedures used for the detection and identification of herpesviruses that infect bivalve molluscs are reviewed. (c) 2006 Elsevier B.V. All rights reserved.
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