FN Archimer Export Format
PT J
TI Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas
BT 
AF GARNIER, Matthieu
   LABREUCHE, Yannick
   GARCIA, Celine
   ROBERT, Maeva
   NICOLAS, Jean-Louis
AS 1:1;2:1;3:2;4:2;5:1;
FF 1:PDG-DOP-DCB-PFOM-PI;2:PDG-DOP-DCB-PFOM-PI;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCB-PFOM-PI;
C1 IFREMER, Ctr Brest, UMR PE2M, F-29280 Plouzane, France.
   IFREMER, LGP, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
   LA TREMBLADE
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
IF 2.558
TC 188
UR https://archimer.ifremer.fr/doc/2007/publication-2406.pdf
LA English
DT Article
AB A study was conducted to investigate the involvement of bacteria in oyster mortalities during summer. Moribund and apparently healthy oysters were sampled during mortality events along the French coast and in rearing facilities, usually when temperature reached 19 degrees C or higher, and oysters were in the gonadal maturation phase. Hemolymph samples were aseptically withdrawn and submitted to bacteriological analysis. In healthy oysters, bacteria colonized hernolymph at low concentrations depending on the location. In most moribund oysters, bacteria were present in hemolymph and other tissues. These bacterial populations were more often diverse in oysters originating from the open sea than from facilities where animals were generally infected by a single type of bacterium. Only the dominant colonies were identified by phenotypic and genotypic characters (RFLP of GyrB gene and partial sequence of 16S rRNA gene). They belonged to a limited number of species including Vibrio aestuarianus, members of the V. splendidus group, V. natriegens, V. parahaemolyticus, and Pseudoalteromonas sp. The most frequently encountered species was V. aestuarlanus (56% of isolates), which was composed of several strains closely related by their 16S rRNA gene but diverse by their phenotypic characters. They appeared intimately linked to oysters. The species within the V. splendidus group were less prevalent (25% of isolates) and more taxonomically dispersed. A majority of the dominant strains of V. aestuarianus and V. splendidus group injected to oysters induced mortality, whereas others belonging to the same species, particularly those found in mixture, appeared innocuous.
PY 2007
PD FEB
SO Microbial Ecology
SN 0095-3628
PU Springer
VL 53
IS 2
UT 000244645600002
BP 187
EP 196
DI 10.1007/s00248-006-9061-9
ID 2406
ER

EF