Subcellular distribution of Ag, Cd, Co, Cu, Fe, Mn, Ph, and Zn in the digestive gland of the common cuttlefish Sepia officinalis

Type Article
Date 2006-12
Language English
Author(s) Bustamante Paco1, 4, Bertrand M2, Boucaud Camou E3, Miramand Pierre1, 4
Affiliation(s) 1 : Univ La Rochelle, Ctr Rech Ecosyst Littoraux Anthropises, UMR 6217, CNRS,IFREMER, F-17042 La Rochelle, France.
2 : Inst Natl Sci & Tech Mer, Conservatoire Natl Arts & Metiers, F-50103 Cherbourg, France.
3 : Univ Caen, Lab Biol & Biotechnol Marines, UMR IFREMER, F-14032 Caen, France.
Source The Journal of Shellfish Research (0730-8000) (National Shellfisheries Association), 2006-12 , Vol. 25 , N. 3 , P. 987-993
WOS© Times Cited 24
Keyword(s) Sepia officinalis, Chromatography, Metallothionein, Detoxification, Trace element, Metal, Cephalopod
Abstract The subcellular fractionation of the digestive gland cells of the common cuttlefish Sepia officinalis was performed to investigate the distribution of metals between organelles and cytosol and the different cytosolic fractions separated by gel-filtration chromatography. Total metal concentrations vary over 3 orders of magnitude, ranging from dwt for Pb to dwt for Zn. With the exception of Cd, Co, and Cu, metals were mostly bound to the organelles. Whereas no specific organelle compartment was found for Mn, Ph, and Zn, Fe was mainly associated with nucleus, brown body, and "boule" fraction (i.e., 52%) and 44% of the total Ag was contained in the lysosomal and mitochondria enriched fraction. The link of metals with hydrosoluble proteins in the cytosolic fraction was investigated at 254 and 280 not. Direct relationship between cytosolic metal and metallothioneins could only be established for Ag and Cu, whereas Cd and Zn seem to mainly bind high (> 70 kDa) and low (< 4 kDa) molecular weight proteins.
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Bustamante Paco, Bertrand M, Boucaud Camou E, Miramand Pierre (2006). Subcellular distribution of Ag, Cd, Co, Cu, Fe, Mn, Ph, and Zn in the digestive gland of the common cuttlefish Sepia officinalis. The Journal of Shellfish Research, 25(3), 987-993. Open Access version : https://archimer.ifremer.fr/doc/00000/2532/