FN Archimer Export Format
PT J
TI Subcellular distribution of Ag, Cd, Co, Cu, Fe, Mn, Ph, and Zn in the digestive gland of the common cuttlefish Sepia officinalis
BT 
AF BUSTAMANTE, Paco
   BERTRAND, M
   BOUCAUD CAMOU, E
   MIRAMAND, Pierre
AS 1:1,4;2:2;3:3;4:1,4;
FF 1:;2:;3:;4:;
C1 Univ La Rochelle, Ctr Rech Ecosyst Littoraux Anthropises, UMR 6217, CNRS,IFREMER, F-17042 La Rochelle, France.
   Inst Natl Sci & Tech Mer, Conservatoire Natl Arts & Metiers, F-50103 Cherbourg, France.
   Univ Caen, Lab Biol & Biotechnol Marines, UMR IFREMER, F-14032 Caen, France.
C2 UNIV LA ROCHELLE, FRANCE
   INSTM, FRANCE
   UNIV CAEN, FRANCE
   IFREMER, FRANCE
SI UNIV-FRANCAISE
SE PDG-DOP-DCN-AGSAE-CRELA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 0.875
TC 27
UR https://archimer.ifremer.fr/doc/2006/publication-2532.pdf
LA English
DT Article
DE ;Sepia officinalis;Chromatography;Metallothionein;Detoxification;Trace element;Metal;Cephalopod
AB The subcellular fractionation of the digestive gland cells of the common cuttlefish Sepia officinalis was performed to investigate the distribution of metals between organelles and cytosol and the different cytosolic fractions separated by gel-filtration chromatography. Total metal concentrations vary over 3 orders of magnitude, ranging from dwt for Pb to dwt for Zn. With the exception of Cd, Co, and Cu, metals were mostly bound to the organelles. Whereas no specific organelle compartment was found for Mn, Ph, and Zn, Fe was mainly associated with nucleus, brown body, and "boule" fraction (i.e., 52%) and 44% of the total Ag was contained in the lysosomal and mitochondria enriched fraction. The link of metals with hydrosoluble proteins in the cytosolic fraction was investigated at 254 and 280 not. Direct relationship between cytosolic metal and metallothioneins could only be established for Ag and Cu, whereas Cd and Zn seem to mainly bind high (> 70 kDa) and low (< 4 kDa) molecular weight proteins.
PY 2006
PD DEC
SO The Journal of Shellfish Research
SN 0730-8000
PU National Shellfisheries Association
VL 25
IS 3
UT 000242839700026
BP 987
EP 993
ID 2532
ER

EF