FN Archimer Export Format
PT J
TI Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions
BT 
AF BOUTET, Isabelle
   LORIN NEBEL, C
   DE LORGERIL, Julien
   GUINAND, B
AS 1:1,2;2:1;3:1,2;4:1;
FF 1:PDG-DOP-DCN-AGSAE-GPIA;2:;3:PDG-DOP-DCM-BOME-LALR;4:;
C1 Univ Montpellier 2, IFREMER, CNRS, UMR 5171,Lab Genome Populat Interact Adaptat, F-34095 Montpellier 5, France.
C2 UNIV MONTPELLIER 2, FRANCE
   IFREMER, FRANCE
SI AUTRE
   MONTPELLIER
SE PDG-DOP-DCN-AGSAE-GPIA
   PDG-DOP-DCM-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 1.391
TC 19
UR https://archimer.ifremer.fr/doc/2007/publication-2537.pdf
LA English
DT Article
DE ;Prolactin;Phylogenctic analysis;MRNA expression;Osmoregulation;Intestine;Gill;Dicentrarchuslabrax
AB dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in gills was significantly greater than in the intestine. Nonetheless, individuals unable to successfully regulate osmotic balance in freshwater presented overall low expression rates. Results are discussed according to the mechanism of sea bass adaptation in the wild and to their life cycle between open sea and lagoons. Finally, a phylogenetic analysis indicated that teleosts are not branched according to their life-history features (e.g. seawater vs. freshwater habitats), and no signature of positive selection was detected across the phylogeny of the prl gene in teleosts. (c) 2006 Elsevier Inc. All rights reserved.
PY 2007
PD MAR
SO Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
SN 1744-117X
PU Elsevier
VL 2
IS 1
UT 000245532700007
BP 74
EP 83
DI 10.1016/j.cbd.2006.12.002
ID 2537
ER

EF