FN Archimer Export Format
PT J
TI Seasonal changes in mRNA encoding for cell stress markers in the oyster Crassostrea gigas exposed to radioactive discharges in their natural environment
BT 
AF FARCY, Emilie
   VOISEUX, Claire
   LEBEL, Jean-Marc
   FIEVET, Bruno
AS 1:1,3;2:1;3:2;4:1;
FF 1:PDG-DOP-DCN-BE-LBEX;2:;3:;4:;
C1 Lab Radioecol Cherbourg Octeville, Inst Radioprotect & Surete Nucl, DEI, SECRE, Octeville, France.
   Univ Caen, IFREMER, Lab Biol & Biotechnol Marines, UMR 100, Caen, France.
C2 IRSN, FRANCE
   UNIV CAEN, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-DOP-DCN-BE-LBEX
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.182
TC 41
UR https://archimer.ifremer.fr/doc/2007/publication-2601.pdf
LA English
DT Article
DE ;Crassostrea gigas;Superoxide dismutase;Metallothionein;Heat shock protein;MRNA;Seasonal variations;Radioactive discharges
AB The North Cotentin area (Normandy, France) hosts several nuclear facilities among which the AREVA reprocessing plant of La Hague is responsible for controlled discharges of liquid radioactive wastes into the marine environment. The resulting increase in radioactivity is very small compared to natural radioactivity. However, concerns about environment protection prompted the scientific community to focus on the effects of the chronic exposure to low concentrations of radionuclides in non-human biota. This study contributes to the evaluation of the possible impact of radioactive discharges on the oyster Crassostrea gigas in the field. Real-time polymerase chain reaction was used to quantify the expression levels of genes involved in cell stress in the oyster. They included members of the heat shock protein family (Hsp70, Hsc72, Hsp90), superoxide dismutase (SOD) and metallothionein (NIT). Times series measurements were built from periodic samplings in the natural environment in order to characterize the natural variability as well as possible seasonal fluctuations. The genes studied exhibited a general seasonal expression pattern with a peak value in winter. The data inversely correlated with seawater temperature and the nature of the relationship between gene expression and temperature is discussed. In parallel, oysters were collected in four locations on the French shores, exposed or not to radioactive liquid wastes from the nuclear facilities hosted in the North Cotentin. The comparison of data obtained in the reference location on the Atlantic coast (not exposed) and data from oysters of the English Channel (exposed) gave no evidence for any statistical difference. However, because of the complexity of the natural environment, we cannot rule out the possibility that other parameters may have masked the impact of radioactive discharges. This dense set of data is a basis for the use of the expression levels of those genes as biomarkers to address the question of the possible effects of chronic exposure of the oyster to low concentrations of radionuclides in controlled laboratory experimental conditions. (c) 2006 Elsevier B.V. All rights reserved.
PY 2007
PD MAR
SO Science of The Total Environment
SN 0048-9697
PU Elsevier
VL 374
IS 2-3
UT 000245364500014
BP 328
EP 341
DI 10.1016/j.scitotenv.2006.11.014
ID 2601
ER

EF