Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis

Type Article
Date 2007-07
Language English
Author(s) Tirape Ana1, Bacque Coralie1, Brizard Raphael2, Vandenbulcke Franck3, Boulo VivianeORCID2
Affiliation(s) 1 : Univ Montpellier 2, UMR5171, IFREMER, CNRS, F-34095 Montpellier 5, France.
2 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
3 : Univ Lille 1, Lab Ecol Numer & Ecotoxicol, EA 3570, F-59655 Villeneuve Dascq, France.
Source Developmental & Comparative Immunology (0145-305X) (Elsevier), 2007-07 , Vol. 31 , N. 9 , P. 859-873
DOI 10.1016/j.dci.2007.01.005
WOS© Times Cited 56
Keyword(s) Rel/NF kappa B pathway, Cell proliferation, Hematopoiesis, Mollusk immunity, Hemocytes, Bivalve mollusk
Abstract The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval stages upon exposure to non-pathogenic bacteria. In early juveniles, a modulation was also observed for Cg-LBP/BPI, Cg-timp, Cg-MyD88 and for Cg-tal, according to the concentration of bacteria. Altogether, the results showed that studying the appearance of immunocompetent cells through their ability to express immune-related genes is a tool to gain insight the ontogenesis of the oyster immune system. (C) 2007 Elsevier Ltd. All rights reserved.
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