FN Archimer Export Format
PT J
TI Diversity and abundance of aerobic and anaerobic methane oxidizers at the Haakon Mosby mud volcano, Barents Sea
BT 
AF LOSEKANN, Tina
   KNITTEL, Katrin
   NADALIG, Thierry
   FUCHS, Bernhard
   NIEMANN, Helge
   BOETIUS, Antje
   AMANN, Rudolf
AS 1:1;2:1;3:2;4:1;5:1,3;6:1,3,4;7:1;
FF 1:;2:;3:PDG-DOP-DCB-EEP-LEP;4:;5:;6:;7:;
C1 Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany.
   IFREMER, Ctr Brest, F-29280 Plouzane, France.
   Alfred Wegener Inst Polar & Marine Res, D-27515 Bremerhaven, Germany.
   Int Univ Bremen, D-28759 Bremen, Germany.
C2 INST MAX PLANCK (MARINE MICROBIOL), GERMANY
   IFREMER, FRANCE
   INST A WEGENER, GERMANY
   UNIV BREMEN, GERMANY
SI BREST
SE PDG-DOP-DCB-EEP-LEP
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 4.004
TC 264
UR https://archimer.ifremer.fr/doc/2007/publication-2617.pdf
LA English
DT Article
AB Submarine mud volcanoes are formed by expulsions of mud, fluids, and gases from deeply buried subsurface sources. They are highly reduced benthic habitats and often associated with intensive methane seepage. In this study, the microbial diversity and community structure in methane-rich sediments of the Haakon Mosby Mud Volcano (HMMV) were investigated by comparative sequence analysis of 16S rRNA genes and fluorescence in situ hybridization. In the active volcano center, which has a diameter of about 500 m, the main methane-consuming process was bacterial aerobic oxidation. In this zone, aerobic methanotrophs belonging to three bacterial clades closely affiliated with Methylobacter and Methylophaga species accounted for 56% +/- 8% of total cells. In sediments below Beggiatoa mats encircling the center of the HMMV, methanotrophic archaea of the ANME-3 clade dominated the zone of anaerobic methane oxidation. ANME-3 archaea form cell aggregates mostly associated with sulfate-reducing bacteria of the Desulfobulbus (DBB) branch. These ANME-3/DBB aggregates were highly abundant and accounted for up to 94% +/- 2% of total microbial biomass at 2 to 3 cm below the surface. ANME-3/DBB aggregates could be further enriched by How cytometry to identify their phylogenetic relationships. At the outer rim of the mud volcano, the seafloor was colonized by tubeworms (Siboglinidae, formerly known as Pogonophora). Here, both aerobic and anaerobic methane oxidizers were found, however, in lower abundances. The level of microbial diversity at this site was higher than that at the central and Beggiatoa species-covered part of the HMMV. Analysis of methyl-coenzyme M-reductase alpha subunit (mcrA) genes showed a strong dominance of a novel lineage, mcrA group f, which could be assigned to ANME-3 archaea. Our results further support the hypothesis of Niemann et al. (54), that high methane availability and different fluid flow regimens at the HMMV provide distinct niches for aerobic and anaerobic methanotrophs.
PY 2007
PD MAY
SO Applied and environmental microbiology
SN 0099-2240
PU American society for microbiology
VL 73
IS 10
UT 000246680500028
BP 3348
EP 3362
DI 10.1128/AEM.00016-07
ID 2617
ER

EF