DNA polymerase switching on homotrimeric PCNA at the replication fork of the euryarchaea Pyrococcus abyssi

Type Article
Date 2007-06
Language English
Author(s) Rouillon Christophe1, Henneke GhislaineORCID1, Flament DidierORCID1, Querellou Joel1, Raffin Jean-Paul1
Affiliation(s) 1 : IFREMER, UMR 6197, Lab Microbiol & Environm Extremes, F-29280 Plouzane, France.
Source Journal of Molecular Biology (0022-2836) (Elsevier), 2007-06 , Vol. 369 , N. 2 , P. 343-355
DOI 10.1016/j.jmb.2007.03.054
WOS© Times Cited 28
Keyword(s) RF C, PCNA loading, DNA polymerase switching, DNA replication, Archaea
Abstract DNA replication in Archaea, as in other organisms, involves large protein complexes called replisomes. In the Euryarchaeota subdomain, only two putative replicases have been identified, and their roles in leading and lagging strand DNA synthesis are still poorly understood. In this study, we focused on the coupling of proliferating cell nuclear antigen (PCNA)loading mechanisms with DNA polymerase function in the Euryarchaea Pyrococcus abyssi. PCNA spontaneously loaded onto primed DNA, and replication factor C dramatically increased this loading. Surprisingly, the family B DNA polymerase (Pol B) also increased PCNA loading, probably by stabilizing the clamp on primed DNA via an essential motif. In contrast, on an RNA-primed DNA template, the PCNA/Pol B complex was destabilized in the presence of dNTPs, allowing the family D DNA polymerase (Pol D) to perform RNA-primed DNA synthesis. Then, Pol D is displaced by Pol B to perform processive DNA synthesis, at least on the leading strand. (C) 2007 Elsevier Ltd. All rights reserved.
Full Text
File Pages Size Access
publication-2638.pdf 39 634 KB Open access
Top of the page