FN Archimer Export Format
PT J
TI Transcriptional regulation of pyruvate kinase and phosphoenolpyruvate carboxykinase in the adductor muscle of the oyster Crassostrea gigas during prolonged hypoxia
BT 
AF LE MOULLAC, Gilles
   BACCA, Helene
   HUVET, Arnaud
   MOAL, Jeanne
   POUVREAU, Stephane
   VAN WORMHOUDT, A
AS 1:1;2:2;3:2;4:2;5:1;6:3;
FF 1:PDG-DOP-DCOP-AQUAPOL-LDHP;2:PDG-DOP-DCB-PFOM-PI;3:PDG-DOP-DCB-PFOM-PI;4:PDG-DOP-DCB-PFOM-PI;5:PDG-DOP-DCB-PFOM-PI;6:;
C1 IFREMER, UMR 100, Argenton En Landunvez, France.
   IFREMER, UMR 100, F-29263 Plouzane, France.
   Museum Natl Hist Nat, Stn Biol Marine, UMR 5178, Concarneau, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   MNHN, FRANCE
SI TAHITI
   BREST
   ARGENTON
SE PDG-DOP-DCOP-AQUAPOL-LDHP
   PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 1.238
TC 33
UR https://archimer.ifremer.fr/doc/2007/publication-2683.pdf
LA English
DT Article
DE ;succinate;alanine;carboxykinase;phosphoenolpyruvate;pyruvate kinase;hypoxia;Oyster,
AB The response of Crassostrea gigas to prolonged hypoxia was investigated for the first time by analyzing the metabolic branch point formed by pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK). PK and PEPCK cDNAs were cloned and sequenced. The main functional domains of the PK sequence, such as the binding sites for ADP/ATP and phosphoenolpyruvate (PEP), were identified whereas the PEPCK sequence showed the specific domain to bind PEP in addition to the kinase-1 and kinase-2 motifs to bind guanosine triphosphate (GTP) and Mg2+, specific for all PEPCKs. A C-terminal extension was detected for the first time in eukaryota PK. Separation of mitochondrial and cytosolic fraction showed that more than 92% of the PEPCK enzyme activity was cytosolic in gills, digestive gland, mantle and muscle. PK and PEPCK mRNAs and enzyme activities have been measured in muscle during prolonged hypoxia for 20 days. Adaptation of PK in hypoxic muscle at transcriptional level occurred lately by decreasing significantly the. PK mRNA level at day 20 while PK enzyme activity was inhibited by the high content of alanine. The PEPCK mRNA ratio in hypoxic muscle significantly increased at day 10 simultaneously to the PEPCK enzyme activity. Succinate accumulation observed at day 10 and day 20 confirmed the anaerobic pathway of muscle metabolism in oyster subjected to hypoxia. Regulation of C. gigas PEPCK in muscle occurred at gene transcription level while PK was first regulated at enzyme level with alanine as allosteric inhibitor, and then at molecular level under a fast effect of hypoxia.
PY 2007
PD JUL
SO Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
SN 1932-5223
PU Wiley InterScience
VL 307A
IS 7
UT 000247658100001
BP 371
EP 382
DI 10.1002/jez.390
ID 2683
ER

EF