FN Archimer Export Format
PT J
TI Potential effects of a low molecular weight Fucoidan extracted from brown algae on bone biomaterial osteoconductive properties
BT 
AF IGONDJO TCHEN CHANGOTADE, S
   KORB, G
   BASSIL, J
   BARROUKH, B
   WILLIG, C
   COLLIEC-JOUAULT, Sylvia
   DURAND, Patrick
   GODEAU, G
   SENNI, K
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:2;8:1;9:1;
FF 1:;2:;3:;4:;5:;6:PDG-DOP-DCB-BM-BMM;7:PDG-DOP-DCB-BM;8:;9:;
C1 Univ Paris 05, Lab Physiopathol Tissus Non Mineralises, Fac Chirurg Dent Montrouge, Paris, France.
   IFREMER, Nantes, France.
C2 UNIV PARIS 05, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-DOP-DCB-BM-BMM
   PDG-DOP-DCB-BM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.706
TC 50
UR https://archimer.ifremer.fr/doc/2007/publication-2695.pdf
LA English
DT Article
DE ;heparan mimetics;biomaterial;bone;osteoblast;Fucoidan
AB In this work we firstly tested the influence of low molecular weight fucoidan extracted from pheophicae cell wall on bidimensional cultured normal human osteoblasts behaviours. Secondly impregnation procedure with LMW fucoidan of bone biomaterial (Lubboc®) we explored in this bone extracellular matrix context its capabilities to support human osteoblastic behaviour in 3D culture. In bidimensionnal cultures we evidenced that: LMW fucoidan promotes human osteoblast proliferation, collagen type I expression and favours precocious alkaline phosphatase activity. Furthermore with LMW fucoidan von Kossa's staining was positive at 30 days and only positive at 45 days in absence of LMW fucoidan. In our three dimensional culture models with the biomaterial pretreated with LMW fucoidan osteoblasts promptly overgrew the pretreated biomaterial. We evidenced too osteoblasts increased proliferation with pretreated biomaterial when compared with untreated biomaterial. On control as well as with LMW fucoidan impregnated biomaterial osteoblasts secreted osteocalcin and expressed BMP2 receptor. In conclusion, in our experimental conditions LMW fucoidan stimulated expression of osteoblastic markers differentiation such as alkaline phosphatase activity, collagen type I expression and mineral deposition, furthermore cell proliferation was favoured. These findings suggest that fucoidan could be clinically useful for bone regeneration and bone substitute design.
PY 2008
PD DEC
SO Journal of Biomedical Materials Research
SN 1549-3296
PU Wiley / Blackwell
VL 87A
IS 3
UT 000260984800012
BP 666
EP 675
DI 10.1002/jbm.a.31819
ID 2695
ER

EF