FN Archimer Export Format
PT J
TI Sequence polymorphism-based identification and quantification of Vibrio nigripulchritudo at the species and subspecies level targeting an emerging pathogen for cultured shrimp in New Caledonia
BT 
AF GOARANT, Cyrille
   REYNAUD, Yann
   ANSQUER, Dominique
   DE DECKER, Sophie
   MERIEN, Fabrice
AS 1:2;2:3;3:2;4:3;5:1;
FF 1:PDG-DOP-DCOP-AQNC;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCOP-AQNC;4:PDG-DOP-DCN-AGSAE-LGP;5:;
C1 Inst Pasteur New Caledonia, Lab Rech Bacteriol, Noumea 988445, New Caledonia.
   IFREMER, Dept Aquaculture, Noumea, New Caledonia.
   IFREMER, Dept Genet & Pathol, La Tremblade, France.
C2 INST PASTEUR, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI SAINT VINCENT
   LA TREMBLADE
SE PDG-DOP-DCOP-AQNC
   PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.153
TC 13
UR https://archimer.ifremer.fr/doc/2007/publication-2729.pdf
LA English
DT Article
DE ;Shrimp;Cluster specific;Vibrio;Pathogen;Mariculture;Real time PCR
AB In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes.   The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the dynamics of this bacterial pathogen in the shrimp farm ecosystem. (C) 2007 Elsevier B.V. All rights reserved.
PY 2007
PD JUN
SO Journal of Microbiological Methods
SN 0167-7012
PU Elsevier
VL 70
IS 1
UT 000248109500004
BP 30
EP 38
DI 10.1016/j.mimet.2007.03.007
ID 2729
ER

EF