FN Archimer Export Format PT J TI Arbitrarily primed PCR to type Vibrio spp. Pathogenic for shrimp BT AF GOARANT, Cyrille MERIEN, F BERTHE, Franck MERMOUD, I PEROLAT, P AS 1:1;2:2;3:3;4:4;5:2; FF 1:PDG-DOP-DCOP-AQNC;2:;3:PDG-DOP-DCN-AGSAE-LGP;4:;5:; C1 Inst Pasteur, Lab Leptospires, Noumea 98845, New Caledonia. IFREMER, Lab Rech Aquacole, Noumea 98846, New Caledonia. CIRAD, EMVT, Programme Elevage, Noumea 98845, New Caledonia. IFREMER, Lab Biol & Ecol Invertebres Marins, F-17390 La Tremblade, France. C2 INST PASTEUR, FRANCE IFREMER, FRANCE CIRAD, FRANCE IFREMER, FRANCE SI NOUMEA LA TREMBLADE SE PDG-DOP-DCOP-AQNC PDG-DOP-DCN-AGSAE-LGP IF 3.389 TC 50 UR https://archimer.ifremer.fr/doc/1999/publication-2734.pdf LA English DT Article AB A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR to the study of vibriosis in penaeid shrimps demonstrates its high discriminating power and the relevance of the epidemiological information provided. This approach would contribute to better knowledge of the ecology of Vibrio spp. and their implication in shrimp disease in aquaculture. PY 1999 PD MAR SO Applied And Environmental Microbiology SN 0099-2240 PU Amer Soc Microbiology VL 65 IS 3 UT 000078882000040 BP 1145 EP 1151 ID 2734 ER EF