FN Archimer Export Format PT J TI Experimental transmission of Marteilia refringens with special consideration of its life cycle BT AF BERTHE, Franck PERNAS, Maria ZERABIB, Maamar HAFFNER, Philippe THEBAULT, Anne FIGUERAS, Antonio AS 1:1;2:2;3:1;4:1;5:1;6:2; FF 1:PDG-DOP-DCN-AGSAE-LGP;2:;3:;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP;6:; C1 IFREMER, France CSIC, Spain C2 IFREMER, FRANCE CSIC, SPAIN SI LA TREMBLADE SE PDG-DOP-DCN-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 IF 1.556 TC 40 UR https://archimer.ifremer.fr/doc/1998/publication-2742.pdf LA English DT Article DE ;Marteilia refringens;Ostrea edulis;transmission trial AB Ever since its first description the paramyxean parasite Marteilia refringens has been recognised as one of the most significant pathogens of bivalve molluscs. The existence of a complex life cycle has been postulated by many authors. However, the transmission route of M. refringens and its life cycle remain unresolved. Experimental observations are presented here that may help lead to a satisfactory hypothesis of the M. refringens life cycle. Trials were performed to test the infectivity of purified early and late stages of M. refringens by different routes of inoculation. Horizontal transmission was also tested by cohabitation with carriers in experimental tanks. Field cohabitation and exposure to natural sources of the parasite helped examine the role of the environment in the transmission of M. refringens. Lastly, naturally infected oysters were monitored in experimental tanks, in order to study infection progression in the oysters through serial samples. These experiments provide no evidence to support direct horizontal transmission of M. refringens, but rather suggest that intermediate or alternative hosts, or free-living stages, are essential in the life cycle of the parasite. PY 1998 PD OCT SO Diseases Of Aquatic Organisms SN 0177-5103 PU Inter-Research VL 34 IS 2 UT 000077346600007 BP 135 EP 144 DI 10.3354/dao034135 ID 2742 ER EF