FN Archimer Export Format
PT J
TI Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia)
BT 
AF ROGIER, Hervé
   HERVIO, Dominique
   BOULO, Viviane
   CLAVIES, C
   HERVAUD, E
   BACHERE, Evelyne
   MIALHE, Eric
   GRIZEL, Henri
   PAU, B
   PAOLUCCI, F
AS 1:1;2:2;3:2;4:1;5:1;6:2;7:2;8:2;9:3;10:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 Sanofi Recherche, Rue du Professeur, Blayac, F-34082 Montpellier, France
   IFREMER, Unite de Recherches en Pathologie, Immunologie et Genetique Moleculaire (URPIGM), B.P. 133, F-17390 La Tremblade, France
   Faculte de Pharmacie, Avenue Charles Flahault, F-34060 Montpellier Cedex, France
C2 SANOFI, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
IN WOS Ifremer jusqu'en 2018
IF 1.556
TC 19
UR https://archimer.ifremer.fr/doc/1991/publication-2756.pdf
LA English
DT Article
AB The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These monoclonal antibodies should prove to be of great value as diagnostic and research tools.
PY 1991
PD AUG
SO Diseases Of Aquatic Organisms
SN 0177-5103
PU Inter-research
VL 11
IS 2
UT A1991GA79600008
BP 135
EP 142
DI 10.3354/dao011135
ID 2756
ER

EF