FN Archimer Export Format PT J TI Preparation and characterization of monoclonal antibodies against neoplastic hemocytes of Mytilus edulis (Bivalvia) BT AF NOEL, Danielle BOULO, Viviane CHAGOT, Dominique MIALHE, Eric PAOLUCCI, Francis CLAVIES, Christine HERVAUD, Eric ELSTON, Ralph AS 1:1;2:1;3:1;4:1;5:2;6:2;7:2;8:3; FF 1:;2:;3:;4:;5:;6:;7:;8:; C1 IFREMER, PATHOL & GENET INVERTEBRES MARINS LAB, BP 133, F-17390 LA TREMBLADE, FRANCE. SANOFI, Service Immunodiagnostic, rue du Professeur Blayac, F-34082 Montpellier, France Battelle, Marine Sciences Laboratory, 439 W. Sequim Bay Road, Sequim, Washington 98382, USA C2 IFREMER, FRANCE SANOFI, FRANCE BATTELLE, USA IN WOS Ifremer jusqu'en 2018 IF 1.556 TC 20 UR https://archimer.ifremer.fr/doc/1991/publication-2757.pdf LA English DT Article AB Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplatic cell antigens were obtained. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when incubated with neoplastic hemocytes, whereas these anbidodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron microscopic visualization. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for establishment of immunoassays for diagnosis and quantification of hemic neoplasia in the mussel. PY 1991 PD MAR SO Diseases Of Aquatic Organisms SN 0177-5103 PU Inter-research VL 10 IS 1 UT A1991FA03400008 BP 51 EP 58 DI 10.3354/dao010051 ID 2757 ER EF