Chemiluminescence analysis of the activity of Pecten maximus hemocytes stimulated with zymosan and host-specific Rickettsiales-like organisms

Type Article
Date 1991-10
Language English
Author(s) Le Gall G1, Bachere Evelyne1, Mialhe Eric1
Affiliation(s) 1 : IFREMER,BIOL & ECOL INVERTEBRES MARINS LAB,UNITE RECH PATHOL IMMUNOL & GENET MOLEC,BP 133,F-17390 LA TREMBLADE,FRANCE.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1991-10 , Vol. 11 , N. 3 , P. 181-186
DOI 10.3354/dao011181
WOS© Times Cited 48
Abstract During a study of Rickettsiales-like organisms (RLO) in Pecten maximus gill, histological observation of free RLO in hemoclymph led us to consider interactions between the pathogen and the hemocytes. Firstly, the production of oxygen radicals by hemocytes was established using a luminol-enhanced chemiluminescence method with zymosan axs stimulant. The effects of serveral inhibitors of oxidative metabolism were investigated. The internalization of RLO into hemocytes was demonstrated by electron microscopy but was not related to chemiluminescent activity. The possible involvement of RLO acid phosphatase as the inhibitor of respiratory burst was assessed.
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Le Gall G, Bachere Evelyne, Mialhe Eric (1991). Chemiluminescence analysis of the activity of Pecten maximus hemocytes stimulated with zymosan and host-specific Rickettsiales-like organisms. Diseases Of Aquatic Organisms, 11(3), 181-186. Publisher's official version : https://doi.org/10.3354/dao011181 , Open Access version : https://archimer.ifremer.fr/doc/00000/2759/